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产品描述：大鼠基质金属蛋白酶13(MMP-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP13含量。MMP13即基质金属蛋白酶 13，在人体组织重塑、胚胎发育等生理过程中发挥作用，异常表达与关节炎、癌症等疾病相关。研究聚焦其在疾病发生发展中的机制，如促进肿瘤侵袭转移等，旨在开发针对性治疗药物。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，适用于探索骨关节炎、肿瘤侵袭转移等疾病模型中基质代谢调控机制，或评估创伤修复、炎症微环境等基础研究中的蛋白酶活性动态本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit; UMRCASE ELISA Kit; Fragment ELISA Kit
缩写：MMP13
Uniprot No.：P23097
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.44 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-90 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-105 | |||||
回收率：
| The recovery of rat MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected60002.2312.2692.2502.13830001.5261.5391.5331.42115000.9740.9970.9860.8747500.5780.5960.5870.4753750.3450.3270.3360.224187.50.2510.2410.2460.13493.750.1850.1890.1870.07500.1110.1130.112 | ||||||
货期：3-5 working days
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