Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E14220Rb

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：兔子基质金属蛋白酶13(MMP-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP13含量。MMP13即基质金属蛋白酶13，是参与细胞外基质代谢的关键酶。其异常表达与多种疾病的发生发展密切相关，如骨关节炎、肿瘤转移等。研究主要围绕其在疾病进程中的作用机制，探索以它为靶点开发疾病治疗药物的可能。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，本试剂盒适用于体外实验研究，可满足关节炎模型构建、骨代谢研究、肿瘤微环境分析等科研场景中MMP - 13的动态监测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MMP13Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit
缩写：MMP13
Uniprot No.：O62806
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.44 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	105
	Range %	97-108
1:4	Average %	90
	Range %	85-95
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rabbit MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-95
EDTA plasma (n=4)	98	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected60002.5542.4442.4992.40530001.9371.9761.9571.86315001.4091.4221.4161.3227500.8150.7890.8020.7083750.4220.4340.4280.334187.50.3020.2890.2960.20293.750.2070.1980.2030.10900.0930.0950.094