首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP2含量。MMP2（基质金属蛋白酶2）是一种降解细胞外基质成分的酶，参与细胞迁移、血管重塑、组织修复、肿瘤侵袭、炎症和动脉粥样硬化等生理和病理过程。其表达受多种因素调控，如生长因子、细胞因子和激素等，并在多种癌症的侵袭和转移中发挥作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于体外科研场景，如细胞迁移实验的分泌水平监测、组织修复模型中的动态表达分析，或炎症相关疾病机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit
缩写：MMP2
Uniprot No.：P33436
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:10 | Average % | 93 | ||||
| Range % | 90-93 | |||||
| 1:20 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:40 | Average % | 96 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of rat MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 103 | 95-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.1942.2832.2392.093201.8041.9591.8821.736101.2801.3861.3331.18750.8910.8700.8810.7352.50.4550.4640.4600.3141.250.2970.3070.3020.1560.6250.2280.2250.2270.08100.1420.1500.146 | ||||||
货期：3-5 working days
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