首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP2含量。MMP2 即基质金属蛋白酶 2，在细胞外基质降解等生理过程中发挥重要作用。其异常表达与肿瘤侵袭、转移、血管生成以及心血管疾病等病理情况密切相关。研究多聚焦于其作用机制，以探索针对 MMP2 的疾病诊断标志物和治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验模型或动物研究中MMP - 2表达水平的动态监测，例如肿瘤微环境分析、组织修复机制研究或炎症相关信号通路探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit
缩写：MMP2
Uniprot No.：P33434
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 87-103 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 82-105 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 81-99 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 87-108 | |||||
回收率：
| The recovery of mouse MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-105 | ||||
| EDTA plasma (n=4) | 95 | 84-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7542.7082.7312.6085001.8431.9111.8771.7542501.1181.0741.0960.9731250.6790.6520.6660.54362.50.3750.3570.3660.24331.20.2920.3080.3000.17715.60.1950.2120.2040.08100.1250.1210.123 | ||||||
货期：3-5 working days
×
