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产品描述：犬基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP2/Gelatinase A含量。MMP2/Gelatinase A即基质金属蛋白酶 2，在细胞外基质降解和重构中起关键作用。其异常表达与肿瘤生长、侵袭、转移等密切相关。研究主要围绕其表达调控机制、与其他分子相互作用等，以探索开发针对它的治疗癌症等疾病的药物。试剂盒检测范围为4.69 ng/mL-300 ng/mL，适用于犬类疾病模型研究中MMP-2动态变化的监测，如肿瘤侵袭转移机制分析、伤口愈合评估或心血管疾病相关基质重塑研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MMP2/Gelatinase A
Uniprot No.：F1PMK7
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：4.69 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-106 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 80-95 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of canine MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-101 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3002.0281.9521.9901.8541501.7361.7761.7561.620751.5021.4521.4771.34137.51.1281.1391.1340.99818.750.7570.7850.7710.6359.380.5030.5240.5140.3784.690.3310.3290.3300.19400.1370.1340.136 | ||||||
货期：3-5 working days
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