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Mouse matrix metalloproteinase 3,MMP-3 ELISA kit

  • 中文名: 小鼠基质金属蛋白酶3(MMP-3)ELISA Kit
货号: IPD-E04678m
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠基质金属蛋白酶3(MMP-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的MMP3含量。MMP3,即基质金属蛋白酶3,是一种参与细胞外基质降解的酶,主要在组织重塑、伤口修复、动脉粥样硬化进展和肿瘤侵袭中发挥作用。其表达受多种刺激物调控,能够降解多种胶原蛋白和蛋白聚糖,并激活其他MMPs,如MMP-1、MMP-7和MMP-9,在结缔组织重塑中起关键作用。试剂盒检测范围为0.312 ng/ml-20 ng/ml,该产品适用于基础研究中的炎症模型评估、组织修复机制探索或疾病相关分子通路分析,能够为小鼠模型中的MMP-3表达水平检测提供可靠数据支持,满足细胞实验、动物模型及生物样本分析的科研需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; EMS-2 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit
  • 缩写:
    MMP3
  • Uniprot No.:
    P28862
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates.
  • 检测范围:
    0.312 ng/ml-20 ng/ml
  • 灵敏度:
    0.078 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    87


    Range %

    83-95


    1:2

    Average %

    97


    Range %

    92-102


    1:4

    Average %

    92


    Range %

    87-96


    1:8

    Average %

    93


    Range %

    87-98


  • 回收率:

    The recovery of mouse MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    88-95


    EDTA plasma (n=4)

    101

    95-108


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    2.367

    2.264

    2.316

    2.157


    10

    1.688

    1.655

    1.672

    1.513


    5

    0.975

    0.999

    0.987

    0.828


    2.5

    0.613

    0.652

    0.633

    0.474


    1.25

    0.346

    0.364

    0.355

    0.196


    0.625

    0.251

    0.266

    0.259

    0.100


    0.312

    0.197

    0.189

    0.193

    0.034


    0

    0.156

    0.162

    0.159




  • 货期:
    3-5 working days

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