首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠基质金属蛋白酶7(MMP-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP7含量。MMP7（基质金属蛋白酶 7）在多种生理和病理过程中发挥重要作用。其它与肿瘤发生、发展及转移密切相关。研究机制上，能降解细胞外基质成分，利于肿瘤细胞侵袭和转移，还可激活多种生长因子，参与肿瘤微环境重塑等，是潜在的癌症治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域对MMP-7表达水平的精准分析。适用于动物实验中对肝纤维化、肺损伤或肿瘤模型等疾病机制研究，帮助科研人员评估组织修复、炎症进展或肿瘤侵袭相关分子事件中MMP-7的动态变化，为体外实验提供可靠的定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp7 ELISA Kit; Mmp-7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
缩写：MMP7
Uniprot No.：P50280
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 81-88 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of rat MMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6122.6372.6252.4705001.7921.7361.7641.6092501.1011.0741.0880.9331250.6030.6340.6190.46462.50.4310.4140.4230.26831.250.3330.3090.3210.16615.60.2320.2190.2260.07100.1560.1530.155 | ||||||
货期：3-5 working days
×
