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产品描述：小鼠金属硫蛋白2(MT-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MT-2含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于小鼠模型中的金属代谢异常、氧化应激相关疾病机制研究，以及重金属暴露实验、肝脏损伤模型或环境毒理学研究中的生物标志物分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mt2 ELISA Kit; Metallothionein-2 ELISA Kit; MT-2 ELISA Kit; Metallothionein-II ELISA Kit; MT-II ELISA Kit
缩写：MT-2
Uniprot No.：P02798
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MT-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 86-93 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 92-97 | |||||
回收率：
| The recovery of mouse MT-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 101-106 | ||||
| EDTA plasma (n=4) | 95 | 92-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10001.9752.0221.9991.8425001.3621.3771.3701.2132500.8110.8620.8370.6801250.4330.4390.4360.27962.50.3040.3300.3170.16031.20.2620.2740.2680.11115.60.2140.2200.2170.06000.1630.1500.157 | ||||||
货期：3-5 working days
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