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产品描述：小鼠粘蛋白/粘液素5B(MUC5B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MUC5B含量。MUC5B是重要的黏液蛋白靶点。其背景在于它在呼吸道黏液分泌中起关键作用，异常表达与多种呼吸道疾病如哮喘、慢性阻塞性肺疾病等相关。研究机制主要聚焦于调控其表达与分泌的信号通路，以探索干预策略，改善疾病黏液高分泌状况。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于体外实验研究，如探究呼吸道疾病模型中黏液分泌调控机制、胃肠黏膜屏障功能评估、肿瘤微环境中黏蛋白表达分析等科研场景，为炎症性疾病、黏膜相关病理生理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MUC5B
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 81-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 93-105 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-96 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.2362.1412.1892.098401.8791.8011.8401.749201.4611.3691.4151.324100.8530.7930.8230.73250.5120.4960.5040.4132.50.3070.3010.3040.2131.250.1950.1860.1910.10000.0930.0880.091 | ||||||
货期：3-5 working days
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