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产品描述：小鼠可溶性肌球蛋白重链1(sMHC-1)酶联免疫试剂盒SA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MYH1含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，为研究肌肉相关疾病模型（如心肌缺血、骨骼肌萎缩等）提供可靠工具；科研人员可通过该产品分析运动生理学中肌肉适应性变化、评估药物或基因干预对肌肉功能的影响，或探索肌肉退行性疾病的分子机制；适用于基础研究、药效评价及生物标志物筛选等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Myh1 ELISA Kit; Myosin-1 ELISA Kit; Myosin heavy chain 1 ELISA Kit; Myosin heavy chain 2x ELISA Kit; MyHC-2x ELISA Kit; Myosin heavy chain ELISA Kit; skeletal muscle ELISA Kit; adult 1 ELISA Kit
缩写：MYH1
Uniprot No.：Q5SX40
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sMHC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 85-99 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-106 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 81-90 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
回收率：
| The recovery of mouse sMHC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-96 | |||||
| EDTA plasma (n=4) | 87 | 92-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.0092.0612.0351.862 5001.4241.4021.4131.240 2500.9800.9510.9660.793 1250.5770.5890.5830.410 62.50.3800.3980.3890.216 31.20.2710.2680.2700.097 15.60.2230.2150.2190.046 00.1760.1700.173 | |||||||
货期：3-5 working days
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