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Human Neural cell adhesion molecule 1(NCAM1) ELISA kit

  • 中文名： 人神经细胞粘附分子1(NCAM1)ELISA kit
货号: IPD-EL015511HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人神经细胞粘附分子1(NCAM1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的NCAM1含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品支持多种生物样本类型，可广泛应用于神经生物学研究、肿瘤微环境分析、细胞信号传导机制探索等科研领域，特别适用于体外神经细胞模型构建、疾病标志物筛选及药物作用机理研究等实验场景，为科研人员提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    antigen MSK39 identified by monoclonal ELISA Kit 5.1H11 ELISA Kit; antigen recognized by monoclonal ELISA Kit 5.1H11 ELISA Kit; CD56 ELISA Kit; cell adhesion molecule, neural, 1 ELISA Kit; MSK 39 ELISA Kit; MSK39 ELISA Kit; N-CAM-1 ELISA Kit; NCAM 1 ELISA Kit; NCAM ELISA Kit; NCAM C ELISA Kit; NCAM-1 ELISA Kit; NCAM1 ELISA Kit; NCAM1_HUMAN ELISA Kit; NCAMC ELISA Kit; Neural cell adhesion molecule 1 ELISA Kit; Neural cell adhesion molecule NCAM ELISA Kit; OTTHUMP00000235666 ELISA Kit
  • 缩写：
    NCAM1
  • Uniprot No.：
    P13591
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human NCAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    93


    Range %

    89-98


    1:2

    Average %

    85


    Range %

    81-92


    1:4

    Average %

    89


    Range %

    82-96


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human NCAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-101


    EDTA plasma (n=4)

    87

    83-92


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.092

    2.134

    2.113

    2.010


    1000

    1.614

    1.667

    1.640

    1.538


    500

    1.140

    1.088

    1.114

    1.011


    250

    0.705

    0.729

    0.717

    0.614


    125

    0.391

    0.381

    0.386

    0.283


    62.5

    0.287

    0.293

    0.290

    0.187


    31.25

    0.221

    0.214

    0.217

    0.115


    0

    0.101

    0.105

    0.103




  • 货期：
    3-5 working days

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