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产品描述：猪内皮型一氧化氮合成酶(eNOS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NOS3含量。NOS3即一氧化氮合酶3，是一种重要的靶点。它能催化生成一氧化氮，在血管舒张、血压调节、抑制血小板聚集等生理过程中发挥关键作用。目前对其研究聚焦于解析其在心血管疾病等发病机制中的作用，以探索潜在治疗策略。试剂盒检测范围为3.12 IU/mL-200 IU/mL，本试剂盒适用于科研领域中对猪模型血管功能研究、疾病机制探索以及药物干预效果评价等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NOS3 ELISA Kit; NOS ELISA Kit; Nitric oxide synthase ELISA Kit; endothelial ELISA Kit; EC 1.14.13.39 ELISA Kit; Constitutive NOS ELISA Kit; cNOS ELISA Kit; EC-NOS ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; NOS type III ELISA Kit; NOSIII ELISA Kit
缩写：NOS3
Uniprot No.：Q28969
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：3.12 IU/mL-200 IU/mL
灵敏度：0.78 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-100 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-108 | |||||
回收率：
| The recovery of pig eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-92 | ||||
| EDTA plasma (n=4) | 100 | 97-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected2002.5892.5472.5682.3861001.8291.8091.8191.637501.0641.0871.0760.894250.6430.6570.6500.46812.50.4500.4680.4590.2776.250.3580.3690.3640.1823.120.2740.2980.2860.10400.1810.1830.182 | ||||||
货期：3-5 working days
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