首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠NOV蛋白同源物(NOV)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NOV含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，可应用于小鼠模型中的NOV蛋白表达水平研究，例如在组织再生、心血管疾病或代谢性疾病相关机制探索中，评估不同处理条件或病理状态下蛋白的动态变化，为分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccn3 ELISA Kit; NovCCN family member 3 ELISA Kit; Cellular communication network factor 3 ELISA Kit; Nephroblastoma-overexpressed gene protein homolog ELISA Kit; Protein NOV homolog ELISA Kit; NovH ELISA Kit
缩写：NOV
Uniprot No.：Q64299
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.85 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOV in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2000 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4000 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:8000 | Average % | 104 | |||||
| Range % | 100-112 | ||||||
回收率：
| The recovery of mouse NOV spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-96 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.7372.6232.6802.5907501.8411.7151.7781.6883751.0601.0041.0320.942187.50.6830.6500.6670.577940.3630.3710.3670.277470.2440.2590.2520.16223.50.1710.1620.1670.07700.0930.0870.090 | |||||||
货期：3-5 working days
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