| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP1B3 |
| Uniprot No | P54709 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-279aa |
| 氨基酸序列 | MTKNEKKSLNQSLAEWKLFIYNPTTGEFLGRTAKSWGLILLFYLVFYGFL AALFSFTMWVMLQTLNDEVPKYRDQIPSPGLMVFPKPVTALEYTFSRSDP TSYAGYIEDLKKFLKPYTLEEQKNLTVCPDGALFEQKGPVYVACQFPISL LQACSGMNDPDFGYSQGNPCILVKMNRIIGLKPEGVPRIDCVSKNEDIPN VAVYPHNGMIDLKYFPYYGKKLHVGYLQPLVAVQVSFAPNNTGKEVTVEC KIDGSANLKSQDDRDKFLGRVMFKITARA |
| 预测分子量 | kDa |
| 蛋白标签 | GST tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP1B3重组蛋白的3篇示例参考文献(内容为虚构示例，仅供格式参考)：
1. **文献名称**: "Structural characterization of recombinant human ATP1B3 subunit and its role in Na+/K+-ATPase assembly"
**作者**: Zhang L, et al.
**摘要**: 本研究成功表达并纯化了重组人ATP1B3蛋白，通过X射线晶体学解析其三维结构，揭示了其与α亚基相互作用的分子机制，证实ATP1B3对Na+/K+-ATP酶复合体稳定性至关重要。
2. **文献名称**: "ATP1B3 regulates cellular migration via modulation of focal adhesion dynamics in cancer cells"
**作者**: Kim S, Patel R.
**摘要**: 通过重组ATP1B3蛋白的体外功能实验，发现其过表达可增强肿瘤细胞迁移能力，并证明该效应与整合素信号通路及黏着斑激酶(FAK)磷酸化水平升高相关。
3. **文献名称**: "High-yield production of bioactive ATP1B3 in E. coli using a SUMO fusion tag system"
**作者**: Müller J, et al.
**摘要**: 开发了一种基于SUMO标签的大肠杆菌重组表达体系，实现了ATP1B3蛋白的高效可溶性表达，纯化产物在体外成功恢复了Na+/K+-ATP酶活性，为功能研究提供可靠工具。
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索关键词"ATP1B3 recombinant protein"获取。
ATP1B3. also known as the β3 subunit of Na+/K+ ATPase, is a critical component of the sodium-potassium pump, a membrane-bound enzyme essential for maintaining cellular electrochemical gradients. This protein belongs to the β-subunit family (β1. β2. β3. β4) that regulates the stability, trafficking, and functional activity of the catalytic α-subunit. Specifically, ATP1B3 facilitates the structural maturation of the α-β heterodimer and modulates ion transport efficiency. While widely expressed in tissues like brain, liver, and immune cells, its dysregulation has been linked to neurological disorders, cancer progression, and metabolic diseases.
Recombinant ATP1B3 protein is engineered using biotechnological platforms (e.g., mammalian expression systems like HEK293 or CHO cells) to produce purified, functional isoforms for research. Its production typically involves cloning the human ATP1B3 gene into expression vectors, followed by transfection, protein purification (affinity chromatography), and validation via Western blot or mass spectrometry. This recombinant tool enables studies on ion pump assembly, cellular homeostasis mechanisms, and disease-specific molecular interactions. Researchers utilize it to investigate ATP1B3's role in tumor metastasis (particularly in glioblastoma and leukemia), neural excitability, and drug resistance. Additionally, it serves as an antigen for antibody development in diagnostic assays. Recent studies also explore its potential as a therapeutic target, given its involvement in pathological calcium signaling and cell adhesion processes. The availability of recombinant ATP1B3 accelerates mechanistic insights into sodium-potassium pump biology and associated pathologies.
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