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产品描述：人神经突蛋白(NRN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NRN1含量。人神经突蛋白(NRN1)是一种神经营养因子，促进神经突生长、树突生长、神经元迁移和突触成熟，参与神经发育、突触可塑性和脊髓轴突再生，在阿尔茨海默症等神经退行性疾病中发挥重要作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于神经生物学基础研究，如神经损伤修复模型、脑组织蛋白表达谱分析，或体外药物干预实验中对NRN1动态变化的监测，为探索神经系统相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Neuritin 1 ELISA Kit; Neuritin ELISA Kit; NRN ELISA Kit; Nrn1 ELISA Kit; NRN1_HUMAN ELISA Kit; OTTHUMP00000015989 ELISA Kit; RP3 380B8.2 protein ELISA Kit
缩写：NRN1
Uniprot No.：Q9NPD7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NRN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 86-98 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-107 | |||||
回收率：
| The recovery of human NRN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-89 | ||||
| EDTA plasma (n=4) | 90 | 86-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5902.7802.6852.49110002.2712.2892.2802.0865001.8251.8761.8511.6572501.3841.3971.3911.1971250.8270.8650.8460.65262.50.5810.6040.5930.39931.250.3570.3680.3630.16900.1960.1920.194 | ||||||
货期：3-5 working days
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