首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经营养因子3(NT-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的NT-3含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础科研中对NT - 3表达水平的动态监测，例如神经退行性疾病模型研究、神经损伤修复机制探索或药物干预效果的体外评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ntf3 ELISA Kit; Ntf-3 ELISA Kit; Neurotrophin-3 ELISA Kit; NT-3 ELISA Kit; HDNF ELISA Kit; Nerve growth factor 2 ELISA Kit; NGF-2 ELISA Kit; Neurotrophic factor ELISA Kit
缩写：NT-3
Uniprot No.：P20181
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 87-109 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-106 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 91-102 | ||||||
回收率：
| The recovery of mouse NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 111 | 100-116 | |||||
| EDTA plasma (n=4) | 100 | 90-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.1792.1072.1431.97720001.5331.5781.5561.39010001.0411.0731.0570.8915000.6550.6780.6670.5012500.4870.5000.4940.3281250.4010.4150.4080.24262.50.2780.2840.2810.11500.1680.1640.166 | |||||||
货期：3-5 working days
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