| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THOC7 |
| Uniprot No | Q6I9Y2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-204aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMGAVTDDEVIRKRLLIDGDGAGDDRRI NLLVKSFIKWCNSGSQEEGYSQYQRMLSTLSQCEFSMGKTLLVYDMNLRE MENYEKIYKEIECSIAGAHEKIAECKKQILQAKRIRKNRQEYDALAKVIQ HHPDRHETLKELEALGKELEHLSHIKESVEDKLELRRKQFHVLLSTIHEL QQTLENDEKLSEVEEAQEASMETDPKP |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于THOC7重组蛋白的模拟参考文献示例(注：以下内容为假设性概括，具体文献需通过学术数据库核实)：
1. **文献名称**: "Recombinant THOC7 protein purification and its role in mRNA export"
**作者**: Chen L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组THOC7蛋白，验证其作为THO复合体亚基参与mRNA核质转运的功能，通过敲低实验证明THOC7缺失导致mRNA滞留细胞核。
2. **文献名称**: "Structural analysis of THOC7 in the TREX complex by cryo-EM"
**作者**: Schmidt HB, et al.
**摘要**: 利用冷冻电镜解析含重组THOC7的TREX复合体三维结构，揭示THOC7与THOC5的相互作用界面，提出其在连接复合体与其他RNA结合蛋白中的构象变化机制。
3. **文献名称**: "THOC7 knockdown impairs hematopoietic stem cell differentiation via aberrant splicing"
**作者**: Gupta R, et al.
**摘要**: 通过重组THOC7蛋白回补实验，证实THOC7缺失导致造血干细胞中剪接因子招募异常，引发pre-mRNA加工缺陷，进而影响红细胞分化通路。
建议通过PubMed或Google Scholar搜索真实文献，关键词："THOC7 recombinant" "THO complex" "mRNA export"。
**Background of THOC7 Recombinant Protein**
THOC7 (THO Complex 7) is a critical subunit of the THO complex, a conserved eukaryotic protein assembly involved in mRNA processing and export from the nucleus to the cytoplasm. The THO complex, comprising THOC1-THOC7 and other associated factors, plays a dual role in transcription elongation and coupling mRNA maturation with nuclear export. THOC7 specifically contributes to the structural integrity of the complex and facilitates interactions with other components of the mRNA export machinery, such as the TREX (Transcription-Export) complex.
Biologically, THOC7 is implicated in maintaining genome stability, regulating gene expression, and supporting cellular differentiation, particularly in hematopoietic (blood-forming) systems. Studies highlight its essential role in early development, as THOC7 deficiency in model organisms leads to embryonic lethality. In humans, mutations or dysregulation of THOC7 are linked to developmental disorders, including microcephaly, intellectual disabilities, and bone marrow failure syndromes, underscoring its importance in cell proliferation and tissue homeostasis.
Recombinant THOC7 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling researchers to study its molecular interactions, post-translational modifications, and functional mechanisms *in vitro*. This tool has advanced investigations into mRNA export defects, cancer biology (e.g., leukemia), and rare genetic disorders. Additionally, recombinant THOC7 serves as a potential therapeutic target or biomarker for diseases associated with transcriptional dysregulation.
Overall, THOC7’s role in bridging transcription and mRNA export, combined with its clinical relevance, makes it a focal point for understanding cellular physiology and disease pathogenesis.
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