| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THAP3 |
| Uniprot No | Q8WTV1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-239aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPKSCAA RQCCNRYSSR RKQLTFHRFP FSRPELLKEW VLNIGRGNFK PKQHTVICSE HFRPECFSAF GNRKNLKHNA VPTVFAFQDP TQQVRENTDP ASERGNASSS QKEKVLPEAG AGEDSPGRNM DTALEELQLP PNAEGHVKQV SPRRPQATEA VGRPTGPAGL RRTPNKQPSD HSYALLDLDS LKKKLFLTLK ENEKLRKRLQ AQRLVMRRMS SRLRACKGHQ GLQARLGPEQ QS |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于THAP3重组蛋白的3篇参考文献示例(注：部分文献信息为模拟概括，实际文献需通过数据库验证)：
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1. **文献名称**: *"Recombinant THAP3 Protein Expression and Its Role in Transcriptional Regulation"*
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组THAP3蛋白，证实其通过结合特定DNA序列调控下游基因转录，并分析了其锌指结构域的关键作用。
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2. **文献名称**: *"Structural and Functional Characterization of the THAP3 Recombinant Protein"*
**作者**: Kim S, Park JH.
**摘要**: 利用哺乳动物表达系统制备了重组THAP3蛋白，通过X射线晶体学解析其三维结构，并发现其与细胞周期调控蛋白的相互作用，提示其在增殖中的潜在功能。
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3. **文献名称**: *"THAP3 Recombinant Protein Purification and Enzymatic Activity Assay"*
**作者**: Gupta R, et al.
**摘要**: 优化了THAP3在昆虫细胞中的重组表达条件，开发了高效纯化方案，并验证其具有核酸内切酶活性，为后续机制研究提供了工具。
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**备注**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索具体文献(关键词：THAP3 recombinant protein)。若研究较少，可扩展检索THAP蛋白家族或相关调控通路。
**Background of THAP3 Recombinant Protein**
THAP3 (THAP domain-containing protein 3) is a member of the THAP protein family, characterized by a conserved N-terminal THAP domain—a zinc-coordinating DNA-binding motif that enables sequence-specific interactions with genomic DNA. This domain plays a critical role in transcriptional regulation, chromatin remodeling, and cell cycle control. THAP3 is implicated in modulating gene expression, particularly in pathways related to cell proliferation, differentiation, and apoptosis. Dysregulation of THAP proteins has been linked to cancers, neurological disorders, and developmental defects.
Recombinant THAP3 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian systems, followed by purification to ensure high purity and bioactivity. Its production enables functional studies to dissect molecular mechanisms, such as DNA-binding specificity, protein-protein interactions, and roles in epigenetic regulation. Researchers utilize THAP3 recombinant protein in assays like electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP), and *in vitro* transcription studies.
Studying THAP3 provides insights into its potential as a therapeutic target. For instance, its involvement in cell cycle checkpoints suggests relevance in oncology, while its regulatory functions in neuronal genes may inform neurodegenerative disease research. Structural analysis of recombinant THAP3 also aids in mapping functional domains and designing inhibitors. Overall, THAP3 recombinant protein serves as a vital tool for unraveling its biological significance and translational applications in human health.
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