| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THAP11 |
| Uniprot No | Q96EK4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313aa |
| 氨基酸序列 | MPGFTCCVPGCYNNSHRDKALHFYTFPKDAELRRLWLKNVSRAGVSGCFSTFQPTTGHRLCSVHFQGGRKTYTVRVPTIFPLRGVNERKVARRPAGAAAARRRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSPSASTAQTAQLQPNLVSASAAVLLTLQATVDSSQAPGSVQPAPITPTGEDVKPIDLTVQVEFAAAEGAAAAAAASELQAATAGLEAAECPMGPQLVVVGEEGFPDTGSDHSYSLSSGTTEEELLRKLNEQRDILALMEVKMKEMKGSIRHLRLTEAKLREELREKDRLLAMAVIRKKHG |
| 预测分子量 | 50.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于THAP11重组蛋白研究的代表性文献(基于公开研究内容概括，非真实文献)：
1. **标题**：THAP11 regulates human embryonic stem cell self-renewal through transcriptional repression of CDX2
**作者**：Campbell, S. et al.
**摘要**：研究利用重组THAP11蛋白揭示其通过结合CDX2基因启动子区域抑制转录，维持胚胎干细胞的自我更新能力，证明THAP11在干细胞多能性调控中的关键作用。
2. **标题**：Structural basis of DNA recognition by the THAP11 zinc-finger protein
**作者**：Lee, J.H. & Kim, D.W.
**摘要**：通过体外表达纯化重组THAP11蛋白并进行晶体结构分析，阐明其THAP结构域与靶DNA序列的结合模式，揭示特异性识别机制及关键氨基酸残基。
3. **标题**：THAP11 interacts with HCF-1 and modulates cell cycle progression in cancer cells
**作者**：Rousseaux, M. et al.
**摘要**：利用重组THAP11蛋白进行免疫共沉淀实验，发现其与宿主细胞因子HCF-1相互作用，并证实该复合物通过调控E2F靶基因影响肿瘤细胞周期进程。
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注：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science检索关键词"THAP11 recombinant protein"获取真实文献。
THAP11 (THAP domain-containing protein 11) is a member of the THAP family of proteins characterized by a conserved N-terminal THAP domain, a zinc-coordinating DNA-binding motif unique to this family. First identified in 2003. THAP11 functions as a sequence-specific transcriptional regulator involved in diverse cellular processes, including cell cycle progression, differentiation, and apoptosis. It binds DNA through its THAP domain, which recognizes an 8-bp consensus motif, and recruits co-regulators via its C-terminal regions to modulate gene expression. Notably, THAP11 interacts with host cell factor complex 1 (HCFC1) and other chromatin modifiers, influencing embryonic development and pluripotency in stem cells.
Biologically, THAP11 plays dual roles in cancer progression. While some studies suggest its tumor-suppressive activity by repressing oncogenes like c-Myc and promoting apoptosis, others link its overexpression to poor prognosis in colorectal, liver, and gastric cancers, indicating context-dependent functions. Its involvement in embryonic stem cell self-renewal through cooperative regulation of pluripotency factors (e.g., OCT4. NANOG) highlights its significance in developmental biology.
Recombinant THAP11 protein, typically produced in bacterial (E. coli) or mammalian expression systems, retains DNA-binding and protein-interaction capabilities. This engineered protein serves as a critical tool for studying THAP11’s molecular mechanisms, including chromatin immunoprecipitation assays, in vitro DNA-protein interaction analyses, and screening for small-molecule modulators. Recent research also explores its potential therapeutic applications, particularly in targeting cancer pathways or enhancing cellular reprogramming efficiency. However, challenges remain in fully elucidating its tissue-specific regulatory networks and resolving conflicting observations about its pro- versus anti-tumorigenic effects.
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