| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THRSP |
| Uniprot No | Q92748 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-146aa |
| 氨基酸序列 | MQVLTKRYPKNCLLTVMDRYAAEVHNMEQVVMIPSLLRDVQLSGPGGQAQAEAPDLYTYFTMLKAICVDVDHGLLPREEWQAKVAGSEENGTAETEEVEDESASGELDLEAQFHLHFSSLHHILMHLTEKAQEVTRKYQEMTGQVW |
| 预测分子量 | 43.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于THRSP重组蛋白的3篇参考文献及其摘要概括：
1. **《Recombinant mouse THRSP enhances hepatic lipogenesis in response to thyroid hormone》**
- 作者：Wang Y, et al. (2009)
- 摘要：研究通过在大肠杆菌中表达重组小鼠THRSP蛋白，发现其能够响应甲状腺激素T3的调控，并在肝脏细胞中促进脂肪酸合成相关基因(如ACC、FAS)的表达，揭示了THRSP在脂代谢中的直接作用机制。
2. **《Structural characterization of human THRSP using recombinant protein expression》**
- 作者：Liu J, et al. (2015)
- 摘要：通过昆虫细胞系统重组表达人源THRSP蛋白，利用X射线晶体学解析其三维结构，发现其具有独特的α-螺旋结构域，并验证了该结构在结合甲状腺激素受体及调控靶基因中的关键作用。
3. **《THRSP interacts with hepatic transcription factors to regulate lipid metabolism》**
- 作者：Smith AD, et al. (2020)
- 摘要：通过重组人THRSP蛋白进行体外互作实验，结合蛋白质组学分析，发现THRSP与SREBP1、PPARγ等脂代谢转录因子存在相互作用，阐明了其在肥胖及胰岛素抵抗中的潜在病理机制。
(注：以上文献信息为示例性概括，实际文献需根据具体数据库检索确认。)
THRSP (Thyroid Hormone Responsive Spot 14 Protein) is a small, nuclear-localized protein primarily involved in lipid metabolism regulation. It was initially identified as a thyroid hormone-responsive gene in the liver, with expression tightly regulated by triiodothyronine (T3) through thyroid hormone receptors. Structurally, THRSP contains an α-helical domain critical for its interaction with transcription factors and co-regulators. The protein plays a key role in de novo lipogenesis by modulating the expression of enzymes like acetyl-CoA carboxylase (ACC) and fatty acid synthase (FASN), particularly in metabolic tissues such as liver, adipose, and mammary glands.
Recombinant THRSP protein is typically produced using bacterial (E. coli) or mammalian expression systems, enabling studies of its molecular mechanisms without endogenous protein interference. The purified protein retains functional properties, including DNA-binding capacity and interaction with nuclear receptors. Researchers utilize recombinant THRSP to investigate its role in metabolic disorders, including obesity, non-alcoholic fatty liver disease (NAFLD), and diabetes. Emerging evidence also links THRSP to cancer progression, particularly in hormone-responsive malignancies, where it may influence tumor cell proliferation and lipid metabolism reprogramming. Its small size (14-18 kDa) and stability make it suitable for structural analysis and high-throughput screening for potential therapeutic modulators. Current studies focus on deciphering its paradoxical roles in different tissues and its potential as a biomarker or therapeutic target in metabolic syndromes.
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