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产品描述：鱼免疫球蛋白IgM酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IgM含量。IgM是人体最早产生的免疫球蛋白，在早期免疫防御中发挥关键作用。它可通过多种机制清除病原体，如激活补体系统介导溶菌等。在研究方面，其作为靶点被用于多种疾病如感染性疾病、自身免疫病的治疗药物研发等。试剂盒检测范围为1.25 μg/mL-50 μg/mL，适用于水产养殖研究、环境毒理学评估及鱼类免疫机制探索，例如监测疫苗免疫后抗体应答、评估水质污染物对免疫功能的影响，或解析疾病模型中的免疫动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgM
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 μg/mL-50 μg/mL
灵敏度：1.25 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-101 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 84-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of fish IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-105 | ||||
| EDTA plasma (n=4) | 95 | 91-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 500.1220.1240.123 250.2130.1990.206 11.50.4010.4270.414 3.750.7450.7610.753 1.251.3751.4021.389 02.5412.5322.537 | ||||||
本试剂盒所含材料：
A 96-well Assay plate --The 96-well plate has been pre-coated with goat-anti-rabbit antibody.
Standard (6 x 0.5 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated IgM (1 x 6 ml) --Bind to the IgM antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
IgM Antibody (1 x 6 ml) --Interact with the IgM.
Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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