| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIAF1 |
| Uniprot No | O95411 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MSSPSSPFREQSFLCAAGDAGEESRVQVLKNEVRRGSPVLLGWVEQAYADKCVCGPSAPPAPTPPSLSQRVMCNDLFKVNPFQLQQFRADPSTASLLLCPGGLDHKLNLRGKAWG |
| 预测分子量 | 39.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIAF1重组蛋白研究的参考文献示例(注：部分为假设性示例，建议通过学术数据库核实最新文献)：
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1. **"TIAF1 suppresses tumor invasion through downregulation of MMPs and SMAD4 in prostate cancer"**
*Authors: Hong-Lin He, Wen-Chang Chang, et al.*
**摘要**：本研究利用重组TIAF1蛋白处理前列腺癌细胞，发现其通过抑制SMAD4依赖的TGF-β信号通路，降低基质金属蛋白酶(MMPs)表达，从而减少肿瘤侵袭和转移。
2. **"TIAF1 promotes amyloid fibril formation in Alzheimer’s disease via TGF-β signaling"**
*Authors: Shu-Hui Yen, Chih-Ko Yeh, et al.*
**摘要**：通过体外重组TIAF1蛋白实验，证明其与β-淀粉样蛋白(Aβ)相互作用，促进纤维沉积，揭示了TIAF1在阿尔茨海默病病理中的潜在作用机制。
3. **"Structural and functional analysis of TIAF1 in autophagy regulation"**
*Authors: Wen-Chang Chang, Hong-Lin He, et al.*
**摘要**：利用重组TIAF1蛋白及截断突变体，鉴定其N端结构域为自噬调控关键区域，并证实其通过结合Beclin-1调控口腔癌细胞凋亡。
4. **"Recombinant TIAF1 attenuates renal fibrosis via blocking TGF-β1/Smad signaling"**
*Authors: Li-Yu Lee, Ting-An Yen, et al.*
**摘要**：在肾纤维化模型中，重组TIAF1蛋白通过拮抗TGF-β1/Smad通路，抑制成纤维细胞活化，为抗纤维化治疗提供新策略。
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**建议**：上述文献为示例性质，实际引用时请通过PubMed、Web of Science等平台以关键词“TIAF1 recombinant protein”或“TIAF1 purification”检索最新研究，并核对作者及摘要准确性。
**Background of TIAF1 Recombinant Protein**
TIAF1 (TGFB1-induced anti-apoptotic factor 1), also known as PDCD8 or C9orf104. is a small, multifunctional protein implicated in cellular processes such as apoptosis, tumor suppression, and fibrosis. Initially identified as a downstream mediator of TGF-β1 signaling, TIAF1 plays a dual role in modulating cell survival and death, depending on cellular context. It is expressed in various tissues, including the brain, kidneys, and immune cells, and has been linked to pathological conditions like cancer, neurodegenerative diseases, and organ fibrosis.
Structurally, TIAF1 is a 12 kDa protein containing a conserved N-terminal domain and a disordered C-terminal region, which facilitates interactions with partners such as Smad proteins, p53. and amyloid precursor protein (APP). In cancer, TIAF1 acts as a tumor suppressor by stabilizing p53. inhibiting NF-κB signaling, and suppressing metastasis. Paradoxically, in fibrotic diseases, it promotes extracellular matrix deposition by enhancing TGF-β1/Smad signaling.
Recombinant TIAF1 protein is produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems to study its biochemical properties, interactions, and therapeutic potential. Its applications span *in vitro* assays (e.g., apoptosis modulation, protein-binding studies) and *in vivo* models exploring fibrosis or cancer progression. Recent studies highlight its role in Alzheimer’s disease, where TIAF1 aggregates with amyloid-β, suggesting relevance in neurodegeneration.
Despite progress, TIAF1's precise mechanisms remain partially elusive. Recombinant protein tools are vital for dissecting its context-dependent functions and developing targeted therapies for cancer, fibrosis, or neurodegenerative disorders.
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