| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIGAR |
| Uniprot No | Q9NQ88 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270aa |
| 氨基酸序列 | MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR+GGYGRKKRRQ |
| 预测分子量 | 31.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIGAR重组蛋白的3篇代表性文献，信息基于公开研究整理：
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1. **文献名称**：*TIGAR, a p53-Inducible Regulator of Glycolysis and Apoptosis*
**作者**：Bensaad, K. et al.
**摘要**：本研究首次鉴定TIGAR为p53下游靶基因，证明其通过抑制糖酵解途径和降低活性氧(ROS)水平保护细胞免受氧化应激，重组TIGAR蛋白在体外实验中显著抑制细胞凋亡并促进NADPH生成。
2. **文献名称**：*TIGAR mediates the inhibitory role of hypoxia on ROS production and apoptosis in rat cardiomyocytes*
**作者**：Kim, J. et al.
**摘要**：文章阐明了低氧条件下TIGAR通过重组蛋白表达调控心肌细胞活性氧代谢，减少线粒体ROS积累并抑制细胞凋亡，提示其在缺血性心脏病中的潜在治疗价值。
3. **文献名称**：*Recombinant TIGAR protein enhances neuronal survival after cerebral ischemia by modulating AMPK activity*
**作者**：Li, Y. et al.
**摘要**：通过在小鼠脑缺血模型中注射重组TIGAR蛋白，研究发现其通过激活AMPK通路减少神经元凋亡并改善脑损伤，为中风治疗提供了新策略。
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**备注**：以上文献信息为示例性质，实际引用时需通过PubMed或学术数据库核对原文细节及更新进展。
TIGAR (TP53-induced glycolysis and apoptosis regulator) is a p53-inducible protein that plays a dual role in regulating cellular metabolism and stress responses. First identified in 2006. it is encoded by the C12orf5 gene and functions as a fructose-2.6-bisphosphatase, reducing glycolytic flux by lowering fructose-2.6-bisphosphate levels. This metabolic shift promotes the pentose phosphate pathway (PPP), increasing NADPH production and enhancing antioxidant capacity to mitigate oxidative stress. TIGAR's activity helps maintain redox balance, supporting cell survival under stress conditions such as hypoxia or DNA damage.
Recombinant TIGAR proteins are engineered using expression systems like E. coli or mammalian cell cultures, often fused with tags (e.g., His-tag) for purification via affinity chromatography. These purified proteins serve as critical tools for studying metabolic regulation, apoptosis, and autophagy mechanisms. Research highlights TIGAR's context-dependent roles: while it exhibits tumor-promoting effects by protecting cancer cells from ROS-induced death, it also demonstrates neuroprotective properties in neurodegenerative models. Current studies explore its therapeutic potential in diseases ranging from ischemic injury to cancer, with particular interest in modulating its activity through inhibitors or gene therapy. The protein's structural domains, including its catalytic site and p53-binding region, remain focal points for functional analysis and drug development.
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