| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIPIN |
| Uniprot No | Q9BVW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-301aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMLEPQEN GVIDLPDYEH VEDETFPPFP PPASPERQDG EGTEPDEESG NGAPVPVPPK RTVKRNIPKL DAQRLISERG LPALRHVFDK AKFKGKGHEA EDLKMLIRHM EHWAHRLFPK LQFEDFIDRV EYLGSKKEVQ TCLKRIRLDL PILHEDFVSN NDEVAENNEH DVTSTELDPF LTNLSESEMF ASELSRSLTE EQQQRIERNK QLALERRQAK LLSNSQTLGN DMLMNTPRAH TVEEVNTDED QKEESNGLNE DILDNPCNDA IANTLNEEET LLDQSFKNVQ QQLDATSRNI TEAR |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TIPIN重组蛋白的参考文献摘要概括，供参考：
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1. **文献名称**: **"Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of replication fork"**
**作者**: Yoshizawa-Sugata N, Masai H
**摘要**: 本研究首次在大肠杆菌中重组表达并纯化了人源TIPIN蛋白，发现其与Timeless蛋白形成稳定复合物，并在体外实验中证明TIPIN通过协调复制叉蛋白的组装，维持DNA复制进程的稳定性。
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2. **文献名称**: **"TIPIN depletion leads to chromosome mis-segregation and checkpoint activation in human cells"**
**作者**: Kemp MG, Ghosh M, Liu G, Leffak M
**摘要**: 利用重组TIPIN蛋白进行免疫共沉淀实验，发现TIPIN通过与Chk1激酶相互作用参与DNA损伤检查点调控。研究揭示了TIPIN缺失导致复制压力下染色体错误分离的分子机制。
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3. **文献名称**: **"Structural insights into the interaction of human Tipin with the replication licensing factor Cdc45"**
**作者**: Smith RW, Richet N, Lin YL, et al.
**摘要**: 通过X射线晶体学解析了重组TIPIN蛋白与Cdc45的复合物结构，阐明二者结合界面及对DNA复制起始的调控作用，为TIPIN维持基因组完整性的结构基础提供了证据。
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**备注**：TIPIN相关研究多聚焦于其与DNA复制/修复网络的相互作用，重组蛋白技术常被用于解析其生化功能及互作机制。如需具体文献链接或补充年份信息，可进一步说明。
**Background of TIPIN Recombinant Protein**
TIPIN (TIMELESS-interacting protein) is a conserved nuclear protein that plays a critical role in maintaining genomic stability during DNA replication. It forms a stable complex with TIMELESS, another replication fork-associated protein, to regulate replication fork progression, checkpoint signaling, and replication stress responses. This TIPIN-TIMELESS heterodimer is essential for the stabilization of replication forks, ensuring faithful DNA synthesis and preventing replication-associated DNA damage.
Structurally, TIPIN contains coiled-coil domains that mediate interactions with TIMELESS and other replication machinery components. It is involved in the activation of the ATR (ataxia-telangiectasia and Rad3-related) kinase pathway, a key signaling cascade that coordinates cell cycle arrest and repair mechanisms in response to replication stress. Dysregulation of TIPIN has been linked to genomic instability, chromosomal aberrations, and diseases such as cancer, highlighting its importance in cellular homeostasis.
Recombinant TIPIN protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for *in vitro* studies. It serves as a tool to investigate DNA replication mechanisms, replication stress responses, and protein interactions within the replisome. Researchers also utilize recombinant TIPIN to screen for potential therapeutic agents targeting replication stress vulnerabilities in cancer cells. Its application extends to structural studies, enabling detailed mechanistic insights into replication fork dynamics and checkpoint regulation.
In summary, TIPIN is a pivotal player in DNA replication fidelity, and recombinant TIPIN protein provides a versatile platform for dissecting its roles in genome maintenance and disease pathology.
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