首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抑瘤素M(OSM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的OSM含量。小鼠抑瘤素M（OSM）是一种多功能细胞因子，属于白细胞介素6家族，具有抑制肿瘤细胞生长、调节炎症反应、刺激造血等作用。其通过JAK-STAT及MAPK信号通路介导生物学效应，参与肝脏再生、肝脏疾病等多种生理病理过程。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域探索OSM在炎症模型、肿瘤发展、器官纤维化等病理生理过程中的动态变化，例如评估疾病动物模型中OSM的表达水平、分析药物或基因干预对OSM分泌的影响，或结合细胞实验研究OSM的信号通路机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Osm ELISA Kit; Oncostatin-M ELISA Kit; OSM ELISA Kit
缩写：OSM
Uniprot No.：P53347
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:40 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:80 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:160 | Average % | 98 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of mouse OSM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.2342.1512.1932.0141001.7151.8071.7611.582501.2341.1541.1941.015250.7790.8130.7960.61712.50.5870.6120.6000.4216.250.3530.3450.3490.1703.120.2710.2570.2640.08500.1830.1750.179 | ||||||
货期：3-5 working days
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