首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血小板衍生生长因子可溶性受体α(PDGFsR-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PDGFRA含量。PDGFRA 即血小板衍生生长因子受体α，在细胞增殖、分化等过程起关键作用。异常激活与多种肿瘤及纤维化疾病相关。研究聚焦其信号通路，通过抑制其活性阻断异常信号传递，为肿瘤和纤维化疾病治疗提供新思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞信号通路研究、动物模型血清标志物分析或组织特异性表达水平检测等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pdgfra ELISA Kit; Platelet-derived growth factor receptor alpha ELISA Kit; PDGF-R-alpha ELISA Kit; PDGFR-alpha ELISA Kit; EC 2.7.10.1 ELISA Kit; Alpha platelet-derived growth factor receptor ELISA Kit; Alpha-type platelet-derived growth factor receptor ELISA Kit; CD140 antigen-like family member A ELISA Kit; Platelet-derived growth factor alpha receptor ELISA Kit; CD antigen CD140a ELISA Kit
缩写：PDGFRA
Uniprot No.：P26618
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGFsR-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of mouse PDGFsR-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
| EDTA plasma (n=4) | 99 | 96-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.7953.0122.9042.787 5002.2142.3042.2592.142 2501.4361.5501.4931.376 1250.8990.9740.9370.820 62.50.4720.4610.4670.350 31.250.3510.3420.3470.230 15.60.2440.2530.2490.132 00.1130.1200.117 | ||||||
货期：3-5 working days
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