首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠戊糖素(Pentosidine)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Pentosidine含量。Pentosidine，即戊糖素，是一种高级糖基化终产物（AGEs），其生成与人体衰老、糖尿病、尿毒症等疾病密切相关。研究显示，乳酸菌发酵物能有效抑制戊糖素生成，具有预防或治疗相关疾病的作用。其机制在于乳酸菌发酵物中的有效成分能够减少戊糖素的生成，从而降低与AGEs相关的疾病风险。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，广泛应用于糖尿病模型研究、衰老相关氧化应激机制探索、代谢性疾病中蛋白质修饰分析等科研领域，为体外实验提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Pentosidine
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:800 | Average % | 99 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of mouse pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4012.4622.4322.25110001.8502.0041.9271.7465001.3521.4321.3921.2112500.8490.8990.8740.6931250.6010.6500.6260.44562.50.4470.4170.4320.25131.250.2850.2740.2800.09900.1830.1780.181 | ||||||
货期：3-5 working days
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