首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠可溶性血小板内皮细胞粘附分子1(sPECAM-1/sCD31)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PECAM1含量。PECAM1即血小板内皮细胞黏附分子-1，在血管生成、炎症反应、止血等生理过程发挥重要作用。其研究机制涉及细胞间黏附、信号转导等，通过与配体结合激活下游信号通路，在肿瘤、心血管疾病等领域是潜在治疗靶点。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于心血管疾病模型研究、炎症反应机制探索、内皮细胞功能评估等科研领域，尤其适用于动脉粥样硬化、血栓形成或血管通透性相关实验中动态监测sPECAM - 1的浓度变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pecam1 ELISA Kit; Pecam ELISA Kit; Pecam-1 ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit
缩写：PECAM1
Uniprot No.：Q08481
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:200 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:400 | Average % | 104 | |||||
| Range % | 100-110 | ||||||
| 1:800 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of mouse sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-96 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected2002.9232.8142.8692.7711002.0592.1482.1042.006501.2391.1591.1991.101250.6870.6970.6920.59412.50.4190.4050.4120.3146.250.2810.2730.2770.1793.120.1840.1860.1850.08700.0950.1010.098 | |||||||
货期：3-5 working days
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