首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人绿脓杆菌外毒素A(PEA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、唾液、组织匀浆样本中的PEA含量。PEA（Proximity Extension Assay，邻位延伸分析技术）是一种结合抗体免疫分析和PCR或NGS技术的蛋白质组定量方法。其原理是利用特异性抗体与目标蛋白结合，通过DNA条形码进行定量，具有高灵敏度和特异性，广泛应用于临床疾病研究和药物研发。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品可应用于基础科研中感染模型建立、宿主 - 病原体互作研究、抗生素疗效评估等场景，例如通过检测不同感染阶段生物样本中的 PEA 浓度变化，揭示毒素释放规律及其与疾病进展的关联性，或用于筛选抑制 PEA 活性的潜在化合物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：PEA
种属：Homo sapiens (Human)
样本类型：serum, saliva, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 87-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 91-105 | |||||
回收率：
| The recovery of human PEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 81-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.9302.9222.9262.845 52.1662.3352.2512.170 2.51.5641.5551.5601.479 1.250.9360.8860.9110.830 0.6250.4600.4240.4420.361 0.3120.2760.2590.2680.187 0.1560.1960.2060.2010.120 00.0820.0800.081 | ||||||
货期：3-5 working days
×
