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产品描述：人蛋白质二硫键异构酶A4(PDIA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PDIA4含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，本产品适用于基础科研中PDIA4相关分子机制探索，包括但不限于内质网应激模型构建、氧化还原信号通路研究以及疾病相关生物标志物筛选等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Calcium binding protein intestinal related ELISA Kit; Endoplasmic reticulum resident protein 70 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; EPR70 ELISA Kit; EPR72 ELISA Kit; ER protein 70 ELISA Kit; ER protein 72 ELISA Kit; ERP 70 ELISA Kit; ERp 72 ELISA Kit; ERp-72 ELISA Kit; ERp70 ELISA Kit; ERp72 ELISA Kit; PDIA 4 ELISA Kit; Pdia4 ELISA Kit; PDIA4_HUMAN ELISA Kit; PDIR ELISA Kit; Protein disulfide isomerase A4 ELISA Kit; Protein disulfide isomerase associated 4 ELISA Kit; Protein disulfide isomerase family A member 4 ELISA Kit; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related) ELISA Kit; Protein disulfide isomerase related protein ELISA Kit; Protein disulfide-isomerase A4 ELISA Kit; Protein ERp 72 ELISA Kit; Protein ERp72 ELISA Kit
缩写：PDIA4
Uniprot No.：P13667
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/ml-1500 pg/ml
灵敏度：5.8 pg/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 96-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 88-93 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of human PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-94 | ||||
| EDTA plasma (n=4) | 104 | 99-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 15001.9601.9771.9691.817 7501.2841.3001.2921.140 3750.7250.7130.7190.567 187.50.4120.4060.4090.257 940.2870.2770.2820.130 470.2200.2150.2180.066 23.50.1840.1780.1810.029 00.1500.1540.152 | ||||||
货期：3-5 working days
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