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产品描述：小鼠蛋白质二硫键异构酶A4(PDIA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PDIA4含量。PDIA4作为靶点有重要研究意义。其参与蛋白质折叠、内质网应激反应等过程。研究围绕其在细胞内的分子作用机制，探索它与疾病发生发展的关系，如在癌症等疾病中可能有异常表达，为药物研发、疾病治疗靶点的确定提供了方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础研究中探索PDIA4在细胞应激反应、内质网功能调控中的分子机制，或用于评估疾病模型动物体内PDIA4表达水平与病理进程的关联性，为蛋白质质量控制网络研究及靶向药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pdia4 ELISA Kit; Cai ELISA Kit; Erp72Protein disulfide-isomerase A4 ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; ER protein 72 ELISA Kit; ERp-72 ELISA Kit; ERp72 ELISA Kit
缩写：PDIA4
Uniprot No.：P08003
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of mouse PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-96 | ||||
| EDTA plasma (n=4) | 95 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6542.5432.5992.417102.1352.0772.1061.92451.3451.3321.3391.1572.50.8240.8430.8340.6521.250.5260.5450.5360.3540.6250.3590.3690.3640.1820.3120.2780.2660.2720.09000.1840.1800.182 | ||||||
货期：3-5 working days
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