首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脯氨酸肽酶(PEPD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PEPD含量。PEPD即脯氨酰内肽酶，在多种生理及病理过程中发挥作用，如参与蛋白质代谢等。研究机制方面，它能特异性切割底物中特定肽键。其异常表达与一些疾病相关，目前科研聚焦于探索其在疾病发生发展中的角色及开发靶向疗法。试剂盒检测范围为93.75 mU/mL-6000 mU/mL，适用于科研场景中PEPD的体外定量分析，可应用于代谢性疾病模型、纤维化机制研究、肿瘤微环境分析等基础实验，通过检测生物样本中酶活性变化为细胞外基质重塑、脯氨酸代谢通路调控等研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aminoacyl L proline hydrolase ELISA Kit; Imidodipeptidase ELISA Kit; MGC10905 ELISA Kit; MGC95081 ELISA Kit; Pep 4 ELISA Kit; Pep4 ELISA Kit; pepD ELISA Kit; PEPD_HUMAN ELISA Kit; Peptidase 4 ELISA Kit; Peptidase D ELISA Kit; Prolidase ELISA Kit; Proline dipeptidase ELISA Kit; X pro dipeptidase ELISA Kit; X-Pro dipeptidase ELISA Kit; Xaa-Pro dipeptidase ELISA Kit
缩写：PEPD
Uniprot No.：P12955
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 mU/mL-6000 mU/mL
灵敏度：23.4 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PEPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:10 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:20 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:40 | Average % | 85 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of human PEPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-103 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected60002.6522.4722.5622.41330002.0871.9762.0321.88315001.2931.2041.2491.1007500.5980.5610.5800.4313750.3790.3570.3680.219187.50.2080.2140.2110.06293.750.1650.1590.1620.01300.1530.1450.149 | ||||||
货期：3-5 working days
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