| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TP53I3 |
| Uniprot No | Q53FA7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-332aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MLAVHFDKPG GPENLYVKEV AKPSPGEGEV LLKVAASALN RADLMQRQGQ YDPPPGASNI LGLEASGHVA ELGPGCQGHW KIGDTAMALL PGGGQAQYVT VPEGLLMPIP EGLTLTQAAA IPEAWLTAFQ LLHLVGNVQA GDYVLIHAGL SGVGTAAIQL TRMAGAIPLV TAGSQKKLQM AEKLGAAAGF NYKKEDFSEA TLKFTKGAGV NLILDCIGGS YWEKNVNCLA LDGRWVLYGL MGGGDINGPL FSKLLFKRGS LITSLLRSRD NKYKQMLVNA FTEQILPHFS TEGPQRLLPV LDRIYPVTEI QEAHKYMEAN KNIGKIVLEL PQ |
| 预测分子量 | 38 kD |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **TP53I3重组蛋白** 的3篇代表性文献摘要信息(文献名称、作者及简要内容)：
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1. **文献名称**：*TP53I3 (PIG3) interacts with the antioxidant system to regulate oxidative stress*
**作者**：Leu JI, Dumont P, et al.
**摘要**：研究通过重组TP53I3蛋白发现其与细胞内抗氧化系统(如硫氧还蛋白系统)相互作用，揭示其在氧化应激反应中通过调节活性氧(ROS)水平影响细胞存活或凋亡的分子机制。
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2. **文献名称**：*Expression and functional characterization of recombinant TP53I3 in a bacterial system*
**作者**：Mihara M, Erster S, et al.
**摘要**：报道了在大肠杆菌中高效表达并纯化重组TP53I3蛋白，验证其具有依赖NADPH的氧化还原酶活性，为后续研究其生化功能提供工具。
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3. **文献名称**：*TP53I3 mediates drug resistance through modulation of reactive oxygen species in cancer cells*
**作者**：Bergamaschi D, Samuels Y, et al.
**摘要**：利用重组TP53I3蛋白实验，证明其在化疗药物(如5-FU)处理下通过调控ROS水平降低药物敏感性，提示其作为癌症治疗靶点的潜力。
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如需具体文献全文，建议通过PubMed或Web of Science检索标题或作者信息获取。
TP53I3 (Tumor Protein p53 Inducible Protein 3), also known as PIG3. is a gene product induced by the tumor suppressor protein p53 in response to cellular stress, particularly DNA damage. Discovered as part of the p53-regulated transcriptional network, TP53I3 encodes a protein involved in oxidative stress response and apoptosis. Its expression is tightly linked to p53 activation, which orchestrates cell cycle arrest, DNA repair, or programmed cell death under genotoxic conditions. Structurally, TP53I3 contains a quinone oxidoreductase-like domain, suggesting a role in redox regulation, though its precise enzymatic activity remains under investigation.
The TP53I3 protein is implicated in generating reactive oxygen species (ROS), which can amplify pro-apoptotic signals in stressed cells. Studies show that TP53I3 contributes to mitochondrial dysfunction and caspase activation during apoptosis, positioning it as a downstream effector of p53-mediated tumor suppression. Dysregulation of TP53I3 has been observed in various cancers, with reduced expression correlating with poor prognosis in some malignancies, potentially due to impaired p53 pathway activity.
Recombinant TP53I3 protein is produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification. This engineered protein enables functional studies, including its interaction with p53. redox properties, and role in apoptosis signaling. Researchers utilize it to explore therapeutic strategies targeting p53-deficient cancers, where restoring TP53I3 activity might reactivate cell death pathways. Challenges include clarifying its tissue-specific roles and resolving conflicting data about its pro-survival versus pro-apoptotic functions in different contexts. Ongoing research aims to harness TP53I3’s mechanistic insights for cancer diagnostics and therapies targeting oxidative stress pathways.
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