关键信息
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基因名
rpsH
- 应用
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别名
rpsH; b3306; JW3268; 30S ribosomal protein S8; Small ribosomal subunit protein uS8
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种属
Escherichia coli
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7W7
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表达区间
2-130aa
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分子量
18 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RpsH is a highly conserved protein that plays a crucial role in the assembly and function of the ribosome in bacteria, particularly in the structure and stability of the ribosomal RNA. Its primary function is to bind to the 30S ribosomal subunit, facilitating the proper folding of rRNA and the assembly of ribosomal proteins, which are essential for protein synthesis. Recent studies have highlighted RpsH's importance not just in ribosome function but also in the stringent response, a mechanism that bacteria use to adapt to nutritional stress by modulating their growth and metabolic activities. Mutations or deletions in the rpsH gene can lead to impaired ribosomal assembly, resulting in growth defects and altered resistance to antibiotics. Due to its fundamental role in bacterial physiology, RpsH has garnered attention as a potential target for novel antimicrobial therapies. Researchers are now focusing on producing recombinant RpsH protein to study its structural properties, interaction with rRNA, and its role in ribosome assembly in greater detail. By understanding the intricacies of RpsH function, scientists aim to explore new avenues for drug development that can combat antibiotic resistance and improve our comprehension of bacterial ribosome biogenesis.
