首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素1β前体(pro-IL1B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的pro-IL1B含量。pro-IL1B，即前白细胞介素-1β，是一种无活性的前体蛋白，由pro-Il-1b基因编码。它在炎症刺激下被激活，裂解为具有生物活性的IL-1β，参与炎症和免疫反应。研究显示，pro-IL1B在肿瘤发生发展中扮演重要角色，通过促进炎症反应和肿瘤血管生成，影响肿瘤的侵袭和转移。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于体外炎症模型构建、免疫调控机制研究、药物抗炎效果评估等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：pro-IL1B
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse pro-IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 106 | |||||
| Range % | 101-113 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-99 | ||||||
| 1:8 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
回收率：
| The recovery of mouse pro-IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-100 | |||||
| EDTA plasma (n=4) | 91 | 85-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected4002.4962.3622.4292.2822001.9511.8411.8961.7491001.3491.3191.3341.187500.9350.8850.9100.763250.6030.6270.6150.46812.50.3670.3740.3710.2246.250.2280.2360.2320.08500.1440.1500.147 | |||||||
货期：3-5 working days
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