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产品描述：鸡甲状旁腺激素(PTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTH含量。PTH即甲状旁腺激素，它主要由甲状旁腺主细胞合成和分泌，在调节钙磷代谢、维持血钙平衡上意义重大。研究发现PTH能通过与受体结合激活信号通路，促进骨代谢及肾脏对钙的重吸收等，其相关机制研究对骨病等治疗有重要潜在价值。试剂盒检测范围为6.25 pg/mL-100 pg/mL，该产品广泛应用于禽类钙代谢机制研究、饲料营养评估、疾病模型构建等科研场景，尤其适用于探究环境因素、遗传变异或药物干预对禽类甲状旁腺功能的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit
缩写：PTH
Uniprot No.：P15743
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-100 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-86 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of chicken PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-98 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 1000.1840.1950.190 500.3250.3500.338 250.5310.5040.518 12.51.0061.0261.016 6.252.1172.2202.169 | ||||||
货期：3-5 working days
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