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产品描述：犬甲状旁腺激素(PTH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTH含量。甲状旁腺激素（PTH）是甲状旁腺主细胞分泌的肽类激素。其可调节钙、磷代谢，维持血钙水平稳定。在研究机制上，PTH作用于骨骼、肾脏等靶器官，通过与受体结合激活信号通路，促进骨钙释放、肾小管对钙重吸收及磷排泄等。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于探索犬钙代谢异常、甲状旁腺相关疾病机制及内分泌调控研究；为科研领域提供高效工具，支持动物模型研究、营养代谢实验及疾病机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
缩写：PTH
Uniprot No.：P52212
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 86-102 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 90-103 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 83-102 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 92-105 | ||||||
回收率：
| The recovery of caninePTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 82-105 | |||||
| EDTA plasma (n=4) | 96 | 90-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.7042.7212.7132.5925002.3352.3472.3412.2202501.9761.9911.9841.8631251.4931.4821.4881.36762.50.9970.9690.9830.86231.20.4820.4760.4790.35815.60.3730.3640.3690.24800.1220.1190.121 | |||||||
货期：3-5 working days
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