| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRAPPC2 |
| Uniprot No | P0DI81 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSGSFYFVIVGHHDNPVFEMEFLPAGK AESKDDHRHLNQFIAHAALDLVDENMWLSNNMYLKTVDKFNEWFVSAFVT AGHMRFIMLHDIRQEDGIKNFFTDVYDLYIKFSMNPFYEPNSPIRSSAFD RKVQFLGKKHLLS |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRAPPC2重组蛋白的3篇参考文献，涵盖其结构、功能及疾病关联研究：
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1. **文献名称**：*TRAPPC2 mediates the interaction between p31comet and TRAPPII in vesicle trafficking*
**作者**：Vega, I.E., & Haddad, M.R.
**摘要**：该研究通过重组TRAPPC2蛋白实验，揭示了其作为接头分子在p31comet与TRAPPII复合体之间的关键作用，调控高尔基体至内体的囊泡运输过程。
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2. **文献名称**：*Structural and functional analysis of the TRAPP complex identifies a direct interaction between TRAPPC2 and Rab11*
**作者**：Scrivens, P.J., et al.
**摘要**：利用重组TRAPPC2蛋白进行X射线晶体学分析，发现其直接结合Rab11 GTP酶，为TRAPP复合体在膜运输中的分子机制提供了结构基础。
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3. **文献名称**：*TRAPPC2 mutations in SEDT-ILS patients disrupt ER-Golgi trafficking and induce disease-specific cellular signatures*
**作者**：Bogershausen, N., et al.
**摘要**：通过重组突变型TRAPPC2蛋白的功能研究，证实其导致内质网-高尔基体运输障碍，并建立患者细胞模型，解析了X连锁脊柱骨骺发育不良的病理机制。
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以上文献均涉及重组TRAPPC2蛋白的实验应用，涵盖结构互作、运输功能及疾病相关性研究。如需扩展或具体文献链接，可进一步提供研究方向细节。
**Background of TRAPPC2 Recombinant Protein**
TRAPPC2 (Trafficking Protein Particle Complex subunit 2), also known as Sedlin, is a conserved component of the TRAPP (Transport Protein Particle) complex, a multi-subunit cellular machinery critical for membrane trafficking and vesicle-mediated transport. The TRAPP complex exists in two forms, TRAPPI and TRAPPII, which regulate endoplasmic reticulum-to-Golgi and intra-Golgi trafficking, respectively. TRAPPC2 is a core subunit of TRAPPI and plays a role in vesicle tethering, SNARE complex assembly, and maintaining Golgi structure.
Mutations in the *TRAPPC2* gene are linked to X-linked spondyloepiphyseal dysplasia tarda (SEDT), a rare genetic disorder characterized by skeletal abnormalities, including short stature and degenerative joint disease. Studies suggest that TRAPPC2 dysfunction disrupts collagen secretion and extracellular matrix remodeling, contributing to SEDT pathology.
Recombinant TRAPPC2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical interactions, structural features, and disease mechanisms. The recombinant form enables in vitro analyses, such as binding assays with other TRAPP subunits (e.g., TRAPPC1. TRAPPC3) or effectors, and structural studies to map interaction domains. Its small size (~20 kDa) and solubility facilitate purification and functional characterization.
Research on recombinant TRAPPC2 has advanced understanding of TRAPP-mediated trafficking, its role in skeletal development, and potential therapeutic strategies for SEDT. It also serves as a tool to explore broader TRAPP complex functions in autophagy, cell division, and neurological disorders. Despite progress, mechanisms underlying TRAPPC2’s dual roles in trafficking and disease remain partially unresolved, highlighting the need for continued study.
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