| 纯度 | >95%SDS-PAGE. |
| 种属 | Escherichia coli |
| 靶点 | TPX |
| Uniprot No | P0A862 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-168aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSQTVHFQGNPVTVANSIPQAGSKAQTFTL VAKDLSDVTLGQFAGKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVV LCISADLPFAQSRFCGAEGLNNVITLSTFRNAEFLQAYGVAIADGPLKGL AARAVVVIDENDNVIFSQLVDEITTEPDYEAALAVLKA |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TPX重组蛋白的模拟参考文献示例(注：以下内容为虚构示例，真实文献请通过学术数据库查询)：
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1. **文献名称**: "Efficient Expression and Purification of Recombinant TPX Protein in E. coli for Functional Studies"
**作者**: Zhang L. et al.
**摘要**: 本研究开发了一种利用大肠杆菌表达系统高效表达TPX重组蛋白的方法。通过优化密码子使用和纯化步骤(如His标签亲和层析)，获得了高纯度的TPX蛋白。功能实验表明，重组TPX在体外能有效促进微管组装，验证了其生物学活性。
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2. **文献名称**: "Structural Insights into TPX Protein Interaction with Microtubules via Cryo-EM Analysis"
**作者**: Tanaka K. et al.
**摘要**: 通过冷冻电镜技术解析了TPX重组蛋白与微管结合的复合物结构，揭示了TPX通过N端结构域特异性识别微管正极的分子机制。该研究为TPX在细胞有丝分裂中调控纺锤体形成的功能提供了结构基础。
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3. **文献名称**: "TPX Recombinant Protein Enhances Drug Delivery Efficiency in Cancer Cell Models"
**作者**: Wang Y. et al.
**摘要**: 探索了TPX重组蛋白作为药物载体的潜力。实验证明，TPX与化疗药物阿霉素偶联后，可显著提高药物在肿瘤细胞内的靶向递送效率，并降低对正常细胞的毒性，提示其在癌症治疗中的应用前景。
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如需真实文献，建议通过PubMed、Web of Science或Google Scholar检索关键词如 **"TPX recombinant protein microtubule"** 或 **"TPX protein expression purification"**。
TPX (Targeting Protein for Xklp2) is a microtubule-associated protein critical for mitotic spindle assembly and regulation during cell division. Initially identified in Xenopus egg extracts, TPX2 (the human homolog) plays a central role in activating Aurora A kinase, a key regulator of mitotic progression. By binding to and relieving Aurora A’s autoinhibitory conformation, TPX2 ensures proper kinase activity, which is essential for centrosome maturation, spindle formation, and chromosome segregation. Dysregulation of TPX2 expression is linked to genomic instability and oncogenesis, making it a biomarker in certain cancers.
Recombinant TPX proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional versions for research and therapeutic applications. These proteins retain critical domains for interactions with microtubules and Aurora A, enabling studies on mitotic mechanisms, drug screening, or cancer biology. Recombinant TPX variants may include tagged forms (e.g., GFP, His-tag) for detection or affinity purification. In drug discovery, TPX-derived peptides or inhibitors targeting TPX-Aurora A interactions are explored for anticancer therapies. Additionally, TPX recombinant proteins serve as tools to investigate spindle assembly in cell-free systems or model organisms. Their controlled production ensures reproducibility in dissecting mitotic errors linked to developmental disorders or tumorigenesis, highlighting TPX’s dual role as a vital cellular component and a translational research target.
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