首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人核受体RORγ(RORC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RORC含量。RORC是重要的药物靶点。它属于维甲酸相关孤儿受体家族，在免疫系统中发挥关键作用。研究聚焦于其调控Th17细胞分化机制，影响多种炎症因子分泌。针对它的靶向治疗有望用于自身免疫性疾病、肿瘤等病症的干预。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研领域中对RORγ蛋白表达的定量分析，包括但不限于免疫调控机制研究、药物干预效果评估、疾病动物模型验证等实验场景；为研究RORγ在炎症性疾病、代谢紊乱及肿瘤微环境中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IMD42 ELISA Kit; MGC129539 ELISA Kit; NR1F3 ELISA Kit; Nuclear receptor ROR gamma ELISA Kit; Nuclear receptor ROR-gamma ELISA Kit; Nuclear receptor RZR gamma ELISA Kit; Nuclear receptor RZR-gamma ELISA Kit; Nuclear receptor subfamily 1 group F member 3 ELISA Kit; RAR related orphan nuclear receptor variant 2 ELISA Kit; RAR related orphan receptor C ELISA Kit; RAR related orphan receptor C, isoform a ELISA Kit; RAR related orphan receptor gamma ELISA Kit; RAR-related orphan receptor C ELISA Kit; Retinoic acid binding receptor gamma ELISA Kit; Retinoid related orphan receptor gamma ELISA Kit; Retinoid-related orphan receptor-gamma ELISA Kit; Rorc ELISA Kit; RORG ELISA Kit; RORG_HUMAN ELISA Kit; RZR GAMMA ELISA Kit; RZRG ELISA Kit; TOR ELISA Kit
缩写：RORC
Uniprot No.：P51449
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RORC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-87 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 83-93 | |||||
回收率：
| The recovery of human RORC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 97-107 | ||||
| EDTA plasma (n=4) | 92 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.4242.2352.3302.2024001.6701.7051.6881.5602001.0491.0551.0520.9241000.6640.7040.6840.556500.4320.3990.4160.288250.2910.2860.2890.16112.50.2150.2010.2080.08000.1250.1300.128 | ||||||
货期：3-5 working days
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