首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述:人核心结合因子α1,CBFA1/RUNX2酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、组织培养上清液样本中的RUNX2含量。RUNX2是一种转录因子,对骨组织形成和重建起重要作用。其研究机制涉及调控间充质干细胞分化、软骨细胞成熟、破骨细胞分化等,与多种疾病如骨折愈合、骨代谢性疾病、肿瘤等密切相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于骨骼生物学基础研究、骨发育异常模型评估、骨质疏松机制探索或骨再生相关药物开发等场景。通过定量分析CBFA1/RUNX2的表达动态,可为骨代谢调控网络研究、疾病模型构建及化合物干预效果评价提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Acute myeloid leukemia 3 protein ELISA Kit; Alpha subunit 1 ELISA Kit; AML3 ELISA Kit; CBF alpha 1 ELISA Kit; CBF-alpha-1 ELISA Kit; CBFA1 ELISA Kit; CCD ELISA Kit; CCD1 ELISA Kit; Cleidocranial dysplasia 1 ELISA Kit; Core binding factor ELISA Kit; Core binding factor runt domain alpha subunit 1 ELISA Kit; Core binding factor subunit alpha 1 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; MGC120022 ELISA Kit; MGC120023 ELISA Kit; Oncogene AML 3 ELISA Kit; Oncogene AML-3 ELISA Kit; OSF 2 ELISA Kit; OSF-2 ELISA Kit; OSF2 ELISA Kit; Osteoblast specific transcription factor 2 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OTTHUMP00000016533 ELISA Kit; PEA2 alpha A ELISA Kit; PEA2-alpha A ELISA Kit; PEA2aA ELISA Kit; PEBP2 alpha A ELISA Kit; PEBP2-alpha A ELISA Kit; PEBP2A1 ELISA Kit; PEBP2A2 ELISA Kit; PEBP2aA ELISA Kit; PEBP2aA1 ELISA Kit; Polyomavirus enhancer binding protein 2 alpha A subunit ELISA Kit; Polyomavirus enhancer-binding protein 2 alpha A subunit ELISA Kit; Runt domain ELISA Kit; Runt related transcription factor 2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; RUNX2 ELISA Kit; RUNX2_HUMAN ELISA Kit; SL3 3 enhancer factor 1 alpha A subunit ELISA Kit; SL3-3 enhancer factor 1 alpha A subunit ELISA Kit; SL3/AKV core binding factor alpha A subunit ELISA Kit; SL3/AKV core-binding factor alpha A subunit ELISA Kit
缩写:RUNX2
Uniprot No.:Q13950
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell culture supernates
检测范围:31.25 pg/mL-2000 pg/mL
灵敏度:7.8 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Epigenetics and Nuclear Signaling
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CBFA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:200 | Average % | 91 | |||||
Range % | 86-95 | ||||||
1:400 | Average % | 102 | |||||
Range % | 97-107 | ||||||
1:800 | Average % | 91 | |||||
Range % | 85-97 | ||||||
1:1600 | Average % | 97 | |||||
Range % | 91-103 |
回收率:
The recovery of human CBFA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 95 | 89-98 | |||||
EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected20001.9571.9991.9781.88710001.5671.6031.5851.4945001.1061.1011.1041.0132500.7190.7320.7260.6351250.4070.4120.4100.31962.50.2830.2980.2910.20031.250.1640.1650.1650.07400.0900.0920.091 |
货期:3-5 working days
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