| 纯度 | >80 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AURKB |
| Uniprot No | Q96GD4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344aa |
| 氨基酸序列 | MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPT AAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSH FIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRI YLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRD IKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMH NEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGA QDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Aurora B kinase is recruited to centromeres by the cooperative action of INCENP and Shugoshin"**
*作者：Honda R.*
**摘要**：该研究通过重组AURKB蛋白与INCENP片段共表达，解析了Aurora B激酶在染色体定位中的分子机制，发现Shugoshin蛋白与INCENP协同调控AURKB的激酶活性与中心体定位。
2. **"Reconstitution of Aurora B kinase activity in vitro using recombinant proteins"**
*作者：Vader G. et al.*
**摘要**：利用重组AURKB、INCENP和Survivin蛋白构建体外复合物，验证了AURKB磷酸化组蛋白H3的功能，并揭示其活性依赖于复合物的完整性及微管张力调控。
3. **"Structural basis of Aurora B activation by INCENP and inhibition by barasertib"**
*作者：Bian Y. et al.*
**摘要**：通过重组AURKB-INCENP复合物的晶体结构分析，阐明了INCENP诱导AURKB构象激活的机制，并利用重组蛋白筛选出抑制剂barasertib的结合模式，为抗癌药物设计提供依据。
4. **"Aurora B phosphorylates spatially distinct targets to regulate survivin binding and cohesion release"**
*作者：Jeyaprakash A.A. et al.*
**摘要**：研究通过重组AURKB蛋白进行体外磷酸化实验，发现其通过磷酸化不同底物(如Cohésin亚基SA2)调控染色体黏连与分离，揭示了AURKB在姐妹染色单体分离中的双重作用。
**Background of AURKB Recombinant Protein**
Aurora Kinase B (AURKB), a member of the Aurora kinase family, is a serine/threonine kinase critical for regulating mitotic progression. It plays essential roles in chromosome alignment, spindle assembly checkpoint signaling, and cytokinesis by phosphorylating key substrates such as histone H3 and Survivin. AURKB functions as part of the chromosomal passenger complex (CPC), which ensures accurate chromosome segregation and genomic stability. Dysregulation of AURKB is strongly linked to chromosomal instability, aneuploidy, and tumorigenesis, making it a potential therapeutic target in cancers.
Recombinant AURKB protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce a highly purified, functional form of the kinase for *in vitro* studies. This protein typically retains catalytic activity, enabling researchers to investigate its biochemical properties, substrate interactions, and inhibition mechanisms. Its applications span enzymatic assays (e.g., kinase activity profiling), high-throughput drug screening for oncology drug discovery, and structural studies (e.g., X-ray crystallography or cryo-EM) to elucidate its activation or inhibitor-binding conformations.
Additionally, recombinant AURKB is used to generate antibodies for detecting endogenous protein levels in disease models or clinical samples. Studies leveraging this tool have revealed insights into checkpoint adaptation, polyploidy, and resistance mechanisms in cancer cells. Despite its utility, challenges remain in mimicking post-translational modifications (e.g., auto-phosphorylation) critical for AURKB activation, necessitating careful optimization of expression systems. Overall, recombinant AURKB serves as a vital resource for advancing both basic mitotic biology and translational cancer research.
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