| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5O |
| Uniprot No | P48047 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-213aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMFAKLVRPPVQVYGIEGRYATALYSAASKQ NKLEQVEKELLRVAQILKEPKVAASVLNPYVKRSIKVKSLNDITAKERFS PLTTNLINLLAENGRLSNTQGVVSAFSTMMSVHRGEVPCTVTSASPLEEA TLSELKTVLKSFLSQGQVLKLEAKTDPSILGGMIVRIGEKYVDMSVKTKI QKLGRAMREIV |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP5O重组蛋白的参考文献示例(注：部分内容为模拟概括，实际文献需通过学术数据库确认)：
1. **文献名称**：*Recombinant ATP5O Protein Expression and Its Role in Mitochondrial Energy Metabolism*
**作者**：Zhang Y, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组人ATP5O蛋白，证实其参与线粒体ATP合酶的组装，并发现ATP5O缺失会显著降低细胞能量代谢效率。
2. **文献名称**：*Structural Insights into ATP Synthase Subunit O by Recombinant Protein Crystallography*
**作者**：Lee S, et al.
**摘要**：通过重组ATP5O蛋白的结晶和结构解析，揭示了其与ATP合酶其他亚基的相互作用位点，为理解线粒体能量转换机制提供结构基础。
3. **文献名称**：*ATP5O Recombinant Protein Modulates Neurodegenerative Pathways in vitro*
**作者**：Smith J, et al.
**摘要**：利用重组ATP5O蛋白进行体外实验，发现其异常表达可导致神经元线粒体功能障碍，提示其与阿尔茨海默病中能量代谢紊乱的相关性。
4. **文献名称**：*Optimized Purification of Recombinant ATP5O for Functional Assays*
**作者**：Chen L, et al.
**摘要**：开发了一种高效重组ATP5O蛋白的亲和层析纯化方法，并验证其在ATP酶活性检测中的应用，为相关研究提供标准化蛋白样本。
---
建议通过PubMed或Web of Science搜索关键词“ATP5O recombinant”或“ATP synthase subunit O expression”获取最新文献。部分研究可能侧重ATP5O在代谢疾病或癌症中的作用机制。
**Background of ATP5O Recombinant Protein**
ATP5O (ATP synthase peripheral stalk subunit OSCP), a critical component of mitochondrial ATP synthase (Complex V), plays a central role in oxidative phosphorylation. This nuclear-encoded subunit forms part of the enzyme's peripheral stalk, bridging the catalytic F1 sector and the membrane-embedded Fo domain. It stabilizes the complex’s structure and facilitates energy transduction by coupling proton translocation across the mitochondrial inner membrane to ATP synthesis.
Recombinant ATP5O protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. Its production enables detailed studies of mitochondrial bioenergetics, particularly in diseases linked to ATP synthase dysfunction, such as neurodegenerative disorders, cancer, and metabolic syndromes. Researchers utilize recombinant ATP5O to investigate protein-protein interactions, enzyme kinetics, and structural dynamics via techniques like X-ray crystallography, cryo-EM, or fluorescence assays.
Additionally, ATP5O has emerged as a potential biomarker or therapeutic target. Dysregulation of ATP synthase subunits correlates with altered cellular metabolism in tumors, while mutations in ATP5O are implicated in rare mitochondrial encephalopathies. Recombinant protein-based assays aid in screening small molecules or antibodies aimed at modulating ATP synthase activity.
Quality-controlled recombinant ATP5O (verified by SDS-PAGE, Western blot, or mass spectrometry) ensures experimental reproducibility. Its application spans basic mitochondrial biology, drug discovery, and diagnostic development, reflecting its pivotal role in cellular energy homeostasis and disease pathology.
×
