| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5H |
| Uniprot No | O75947 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-161aa |
| 氨基酸序列 | AGRKLALKTIDWVAFAEIIPQNQKAIASSLKSWNETLTSRLAALPENPPAIDWAYYKANVAKAGLVDDFEKKFNALKVPVPEDKYTAQVDAEEKEDVKSCAEWVSLSKARIVEYEKEMEKMKNLIPFDQMTIEDLNEAFPETKLDKKKYPYWPHQPIENL |
| 预测分子量 | 45.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP5H重组蛋白的3篇示例参考文献(内容为模拟生成，实际文献需进一步检索确认)：
1. **文献名称**: "Structural and functional characterization of human ATP5H in mitochondrial ATP synthase"
**作者**: Zhang Y, et al.
**摘要**: 该研究解析了ATP5H重组蛋白的晶体结构，揭示了其在ATP合酶复合体中的亚基相互作用界面，并通过体外酶活实验证明ATP5H对质子通道的稳定性具有关键调控作用。
2. **文献名称**: "Recombinant ATP5H overexpression mitigates mitochondrial dysfunction in a Parkinson's disease cell model"
**作者**: Lee S, et al.
**摘要**: 利用重组ATP5H蛋白在帕金森病细胞模型中进行了功能验证，发现其过表达可恢复线粒体膜电位并减少活性氧(ROS)积累，提示ATP5H可能作为潜在治疗靶点。
3. **文献名称**: "ATP5H mutations impair oxidative phosphorylation and cause a novel mitochondrial encephalomyopathy"
**作者**: Thompson R, et al.
**摘要**: 通过重组蛋白表达和患者来源的成纤维细胞实验，发现ATP5H基因突变导致ATP合酶组装异常，引发线粒体能量代谢缺陷，首次将该亚基与遗传性神经肌肉疾病相关联。
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如需真实文献，建议在PubMed或Web of Science中以“ATP5H recombinant protein”或“ATP5H subunit function”为关键词检索，重点关注涉及结构解析、疾病机制或治疗应用的论文。
**Background of ATP5H Recombinant Protein**
ATP5H, also known as ATP synthase subunit d, is a nuclear-encoded component of the mitochondrial ATP synthase complex (Complex V), which plays a central role in cellular energy production. This enzyme catalyzes the synthesis of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate during oxidative phosphorylation, utilizing the proton gradient across the mitochondrial inner membrane. ATP5H is a critical subunit of the Fo sector of ATP synthase, contributing to the structural stability and proton translocation activity of the complex.
The ATP5H protein is highly conserved across eukaryotes, underscoring its essential role in mitochondrial function. Dysregulation or mutations in ATP5H have been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, as impaired ATP synthesis disrupts cellular energy homeostasis. Recombinant ATP5H protein is produced through genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications.
Researchers utilize ATP5H recombinant protein to study ATP synthase assembly, function, and interactions within the electron transport chain. It also serves as a tool for investigating mitochondrial diseases, screening potential therapeutic compounds, or developing antibodies for diagnostic applications. Structural studies of recombinant ATP5H have provided insights into its role in proton channel regulation and its contribution to the rotational mechanism of ATP synthase.
Overall, ATP5H recombinant protein is a vital resource for advancing our understanding of mitochondrial bioenergetics and its implications in health and disease.
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