| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSHb |
| Uniprot No | P01222 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-132aa |
| 氨基酸序列 | FCIPTEYTMHIERRECAYCLTINTTICAGYCMTRDINGKLFLPKYALSQDVCTYRDFIYRTVEIPGCPLHVAPYFSYPVALSCKCGKCNTDYSDCIHEAIKTNYCTKPQKSY |
| 预测分子量 | 28.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSHβ重组蛋白的3篇代表性文献，信息基于公开研究整理：
1. **文献名称**：Production and characterization of recombinant human thyroid-stimulating hormone (TSH) in Chinese hamster ovary cells
**作者**：Cole ES, et al.
**摘要**：该研究利用CHO细胞系统重组表达人TSH蛋白，优化了α和β亚基的共表达策略，实现了高产量分泌。纯化的重组TSH表现出与天然激素相似的生物活性，成功应用于甲状腺癌患者的诊断治疗。
2. **文献名称**：Efficient expression and purification of biologically active human thyrotropin (TSH) in HEK293 cells
**作者**：García-Jiménez C, et al.
**摘要**：开发了一种基于HEK293哺乳动物细胞的重组TSH生产体系，通过亲和层析获得高纯度产物。体外实验证实其可有效激活TSH受体，提示在甲状腺功能检测试剂开发中的潜在应用价值。
3. **文献名称**：Bacterial expression of single-chain TSHβ subunit for antibody generation
**作者**：Santos CL, et al.
**摘要**：研究利用大肠杆菌表达系统成功制备了单链TSHβ重组蛋白，通过包涵体复性获得可溶性产物。该蛋白作为免疫原成功诱导了特异性抗体生成，为TSH检测试剂的开发提供了经济高效的抗原来源。
注：实际文献检索建议通过PubMed/Google Scholar使用关键词"recombinant TSH beta subunit"获取最新研究，部分研究可能涉及专利或临床前数据。
**Background of TSHβ Recombinant Protein**
Thyroid-stimulating hormone (TSH), also known as thyrotropin, is a glycoprotein hormone critical for regulating thyroid function. It is composed of two non-covalently linked subunits: an α-subunit (common to other pituitary hormones like LH, FSH, and hCG) and a unique β-subunit (TSHβ) that confers biological specificity. The TSHβ subunit is essential for binding to the TSH receptor (TSHR) on thyroid follicular cells, triggering intracellular signaling pathways that stimulate thyroid hormone synthesis and secretion.
Recombinant TSHβ protein is produced using biotechnological methods, typically through expression in mammalian cell systems (e.g., CHO or HEK293 cells) or bacterial systems (e.g., *E. coli*), followed by purification. Its production enables detailed study of TSH structure-function relationships, receptor interactions, and immune responses in autoimmune thyroid disorders like Graves' disease. Researchers also use recombinant TSHβ to develop diagnostic assays, such as detecting TSH receptor antibodies, or to explore therapeutic applications, including TSH suppression strategies in thyroid cancer. Additionally, it serves as a critical tool in studying TSHβ gene mutations linked to congenital hypothyroidism. The recombinant form offers advantages over animal-derived TSH, including higher purity, consistency, and reduced ethical concerns. Ongoing research continues to explore its potential in personalized medicine and targeted therapies for thyroid-related pathologies.
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