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产品描述：小鼠分泌型免疫球蛋白A(sIgA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、支气管肺泡灌洗液样本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫系统的主要效应分子。能够在黏膜表面阻止病原体黏附与入侵，发挥抗感染等作用。研究机制主要围绕其生成、分泌过程，以及与病原体相互作用等方面，以探索其在疾病防治中的应用潜力。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于科研场景中黏膜免疫相关动物模型的机制研究，例如呼吸道感染模型中局部免疫应答的动态监测、肠道炎症疾病中黏膜屏障功能的评估，或药物/益生菌干预后sIgA分泌水平的定量分析。其宽泛的样本兼容性可支持多维度实验设计，尤其适用于支气管肺泡灌洗液等低浓度样本的检测需求，为黏膜免疫调控机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：sIgA
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, bronchoalveolar lavage fluid
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:40 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:160 | Average % | 98 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of mouse sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.4862.4662.4762.3125001.7721.8411.8071.6432501.2321.2221.2271.0631250.7600.7480.7540.59062.50.4640.4810.4730.30931.20.3250.3120.3190.15515.60.2390.2290.2340.07000.1660.1620.164 | ||||||
货期：3-5 working days
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