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产品描述:牛转化生长因子β2(TGFβ2)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的TGFB2含量。TGFB2 即转化生长因子β2,是 TGF - β超家族成员。它在胚胎发育、组织修复、免疫调节等过程中发挥关键作用。研究发现其异常表达与肿瘤进展、纤维化疾病密切相关,机制涉及细胞增殖、分化、迁移及细胞外基质合成等调控。试剂盒检测范围为6.25 ng/mL-100 ng/mL,适用于动物模型研究、疾病机制分析(如炎症反应、纤维化或肿瘤微环境研究)以及生物标志物筛选等科研场景,支持从低丰度到高浓度样本的稳定检测,为探索牛源生物样本中TGFβ2的表达调控及其功能研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:TGFB2 ELISA Kit; Transforming growth factor beta-2 proprotein ELISA Kit; Milk growth factor ELISA Kit; MGF) [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
缩写:TGFB2
Uniprot No.:P21214
种属:Bos taurus (Bovine)
样本类型:serum, plasma, tissue homogenates
检测范围:6.25 ng/mL-100 ng/mL
灵敏度:6.25 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TGFβ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 85 | ||||
Range % | 82-89 | |||||
1:2 | Average % | 83 | ||||
Range % | 80-86 | |||||
1:4 | Average % | 97 | ||||
Range % | 93-100 | |||||
1:8 | Average % | 96 | ||||
Range % | 91-101 |
回收率:
The recovery of bovine TGFβ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 89 | 87-93 | ||||
EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2Average 1000.2040.1980.201 500.3560.3580.357 250.5920.5720.582 12.51.1071.1051.106 6.252.3322.3692.351 |
货期:3-5 working days
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