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产品描述：猪转化生长因子β1(TGF-β1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TGFB1含量。TGFB1是重要的细胞因子，在胚胎发育、组织修复与再生等生理过程中发挥关键作用。其异常激活与肿瘤、纤维化疾病等多种病理状态密切相关。研究发现它可通过多种信号通路调控细胞生长、分化和迁移，是极具潜力的治疗靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于畜牧科研、动物模型构建、疫苗效力评估等场景，例如通过分析实验性感染、营养干预或药物处理后猪体内TGF-β1的浓度变化，揭示其调控机制或疾病关联性本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写：TGFB1
Uniprot No.：P07200
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-97 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of mouse TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.6542.6362.6452.515 252.3182.4552.3872.257 12.51.7561.8861.8211.691 6.250.9971.0010.9990.869 3.120.5420.5120.5270.397 1.560.3010.3090.3050.175 0.780.2280.2380.2330.103 00.1290.1310.130 | ||||||
货期：3-5 working days
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