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产品描述：鱼转化生长因子β1(TGFB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TGFB1含量。TGFB1 即转化生长因子 -β1，在细胞生长、分化、凋亡等多种生物学进程中发挥关键作用。其异常表达与肿瘤、纤维化等多种疾病密切相关。研究机制主要聚焦于其信号通路，通过调节下游基因表达影响细胞行为，为相关疾病治疗提供潜在靶点。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于科研领域探究环境胁迫、病原感染或营养代谢对鱼类免疫系统的影响，也可用于水产养殖中鱼类健康状态评估及免疫相关分子通路研究，为水生动物免疫学、病理学及生态毒理学研究提供可靠的分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TGFB1
种属：Fish
样本类型：serum, plasma
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 88-95 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 85-105 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 90-102 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-105 | ||||||
回收率：
| The recovery of fish TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 82-98 | |||||
| EDTA plasma (n=4) | 94 | 85-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected4002.7452.7232.7342.6102002.4932.4512.4722.3481001.8941.9141.9041.780501.2711.2861.2791.155250.7160.7020.7090.58512.50.4580.4670.4630.3396.250.2890.2960.2930.16900.1250.1230.124 | |||||||
货期：3-5 working days
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