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产品描述：马转化生长因子β1(TGFB1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的TGFB1含量。TGFB1 即转化生长因子 -β1，在细胞生长、分化、凋亡等过程发挥关键作用。其过度表达与多种疾病如肿瘤、纤维化等相关。研究机制聚焦于其信号通路，探索如何阻断异常激活以研发针对性药物，为疾病治疗提供新策略。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研场景中探索马类动物模型在纤维化疾病、免疫抑制或创伤修复等研究领域的生物标志物动态变化，为兽医研究或比较医学提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写：TGFB1
Uniprot No.：O19011
种属：Equus caballus (Horse)
样本类型：serum, plasma
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:200 | Average % | 103 | |||||
| Range % | 99-108 | ||||||
| 1:400 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
| 1:800 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of horse TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 82-90 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average2000.1260.1310.1291000.2280.2130.221500.3840.3960.390250.6970.6590.67812.50.9080.8720.8906.251.2331.2841.2593.121.4641.5071.48602.0992.2152.157 | |||||||
货期：3-5 working days
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